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phrG [2019-06-24 09:59:06]
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phrG [2019-06-24 09:59:06]

response regulator aspartate phosphatase (RapG) regulator
locus
BSU40310
pI
9.02
mw
4.04 kDa
protein length
38 aa Sequence Blast
gene length
117 bp Sequence Blast
function
control of DegU activity
product
phosphatase (RapG) regulator
essential
no
synonyms
yycL
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.3|Control of response regulators/ other]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.8|Quorum sensing]
  • [category|SW 6|Groups of genes] → [category|SW 6.8|Short peptides]

    • Gene

    Coordinates
    4,141,358 4,141,474

    The protein

    Catalyzed reaction/ biological activity

  • binds to [protein|E1744329B6489F989F93F3E71E51E772E3926ABF|RapG], this results in the inability of [protein|E1744329B6489F989F93F3E71E51E772E3926ABF|RapG] to interact with [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU] [Pubmed|12950930]

    • Protein family

  • phr family (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|E1744329B6489F989F93F3E71E51E772E3926ABF|rapG]-[gene|3B4E60C698B8E4FA286E06B934EC0CEDD5F516A5|phrG]
    description
    [Pubmed|12950930]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16553878], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [Pubmed|12950930], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]

    • regulatory mechanism

  • [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]: repression, [Pubmed|16430695], in [regulon|5A6FBAE6553343092862CB79E150F934978C32A9|SinR regulon]
  • [protein|972401B6AF56EC67FE6F1C3FEA9E2A9FC77C7672|RghR]: repression, [Pubmed|16553878], in [regulon|972401B6AF56EC67FE6F1C3FEA9E2A9FC77C7672|RghR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|12850135], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

    • regulation

  • repressed by glucose (4.8-fold) ([protein|search|CcpA]) [Pubmed|12850135]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-B822 (yycL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1821 NBRP B. subtilis, Japan]
  • BKE40310 ([gene|3B4E60C698B8E4FA286E06B934EC0CEDD5F516A5|phrG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE40310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGCGCCAATCAGAAATCTTT, downstream forward: _UP4_TAAATGAAAAACCCCCGCGG
  • BKK40310 ([gene|3B4E60C698B8E4FA286E06B934EC0CEDD5F516A5|phrG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK40310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGCGCCAATCAGAAATCTTT, downstream forward: _UP4_TAAATGAAAAACCCCCGCGG

    • References

  • 16553878,12850135,12950930